重要规格表
| Detection Methods |
|---|
| Microscopy |
| Description | |
|---|---|
| Overview | Useful for the detection of proliferating cells within fixed tissue samples or cell populations. Requires metabolic incorporation of BrdU (Cat. No. 203806) into sample prior to paraffin-embedding. |
| Catalogue Number | HCS30 |
| Brand Family | Calbiochem® |
| Materials Required but Not Delivered | • Phosphate buffered saline (PBS) • Quenching solution for endogenous peroxidase • Alcohol and xylene • Coverslips • BrdU (i.e. Calbiochem Cat. No. 203806) |
| References | |
|---|---|
| References | Zhang, Q. and Paria, B.C. 2006. Endocrinology 147, 2215. Fukuda, K ., et al. 1990. Anal. Quant. Cytol. Histol. 12, 135. Cattoretti, G. et al. 1993. J. Pathol. 171, 83. Schutte, B., et al. 1987. J. Histochem. & Cytochem. 35,1343. Gonchoroff , N.J., et al. 1986. J. Immunol. Meth. 93, 97. Ellwart, E. and Dormer, P. 1985. Cytometry 6, 513. Gratzner, H.G., et al. 1975. Expl. Cell Res. 95, 88. |
| Product Information | |
|---|---|
| Detection method | Microscopy |
| Form | 50 Tests |
| Kit contains | Trypsin concentrate, trypsin diluent, denaturing solution, blocking solution, biotinylated mouse anti-BrdU, streptavidin-peroxidase, substrate buffer reaction mix, DAB concentrate, hematoxylin, mounting media, control slides (1 stained and 4 unstained), and a user protocol. |
| Positive control | Any cell or tissue sample labeled with BrdU |
| Biological Information | |
|---|---|
| Assay time | ~2.5 hours plus labeling time |
| Sample Type | Paraffin-embedded tissues or fixed cells |
| Safety Information | |
|---|---|
| R Phrase | R: 25-36/37/38-42/43-61 Toxic if swallowed. Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact. May cause harm to the unborn child. |
| S Phrase | S: 24-26-36/37/39-45 Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
| Storage and Shipping Information | |
|---|---|
| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | -20°C |
| Storage Conditions | Upon arrival store the entire contents of the kit at -20°C. Avoid contact with DAB; wearing of latex or rubber gloves is recommended. |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
| Supplemental Information | |
|---|---|
| Kit contains | Trypsin concentrate, trypsin diluent, denaturing solution, blocking solution, biotinylated mouse anti-BrdU, streptavidin-peroxidase, substrate buffer reaction mix, DAB concentrate, hematoxylin, mounting media, control slides (1 stained and 4 unstained), and a user protocol. |
