millipore,
密理博,HCMP1MAG-19K,MILLIPLEX MAP Human Complement Panel 1 - Immunology Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| Complement C2, Complement C4b, Complement C5, Complement C5a, Complement C9, Adipsin, Mannose-Binding Lectin (MBL), Complement Factor I
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HCMP1MAG-19K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Complement Panel 1 - Immunology Multiplex Assay |
| Overview | EMD millipore’s MILLIPLEX® MAP Complement Panel 1 Magnetic Bead Panel is an 8-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: Complement 2, Complement C4B, Complement C5, Complement C5a, Complement C9, Adipson/Complement Factor D, Mannose-Binding Lecting (MBL) and Complement Factor I.
MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior(e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.
Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel
EMD millipore’s MILLIPLEX® MAP Complement Panel 1 Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation. |
| Background Information | The Complement System consists of a large number of plasma proteins that assist, or complement, the ability of phagocytic cells and antibodies to clear pathogens. Although it is part of the innate immune system, not adaptable and unchanging over the course of a lifetime, it can be recruited and activated by the adaptive immune system. The system protein and glycoproteins that are synthesized primarily by hepatocytes normally circulate as inactive pro-proteins. Once stimulated the end result of the cascade is a massive amplification of response and activation of the Membrane Attack Complex (MAC), which forms a transmembrane channel causing osmotic lysis of target cells.
The Complement System consists of three different simultaneous pathways. The classical pathway is stimulated by antigen-antibody complexes; the alternative pathway spontaneously activates on contact with pathogenic cell surfaces; and the mannose-binding lectin pathway recognizes mannose sugars usually present only on pathogenic cell surfaces.
Because of the potential to be extremely damaging to host tissues, Complement System activation must be tightly controlled. The regulating complement control proteins, including CD59, also known as protectin, are present at a higher concentration in the blood plasma than the complement proteins themselves.
The Complement System is thought to play a key role in many diseases with an immune component, such as asthma and many autoimmune diseases, including SLE, IBD and multiple sclerosis. It is also becoming increasingly associated with neurological disease, such as Alzheimer’s disease and conditions such as spinal cord injuries. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: 20
- Intra-assay: 10
|
| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200
|
| Applications
|
| Application | The Human Complement Panel 1 Bead-Based Multiplex Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of complement proteins and factors in human serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | • This is an overnight or two-hour incubation assay.
• This assay requires 25 µl of 1:200 diluted plasma or serum. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.
• Note: To avoid complement activation in samples, which will affect the results, samples should be thawed as rapidly as possible, put on ice and assayed immediately. |
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
- Complement C2
- Complement C4b
- Complement C5
- Complement C5a
- Complement C9
- Adipsin
- Mannose-Binding Lectin (MBL)
- Complement Factor I
|
| Cross Reactivity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Accuracy |
- Complement C2: 70%
- Complement C4b: 87%
- Complement C5: 96%
- Complement C5a: 77%
- Complement C9 65%
- Adipsin (Complement Factor D): 100%
- Mannose-Binding Lectin (MBL): 96%
- Complement Factor I: 95%
|
| Physicochemical Information
|
| Sensitivity |
- Complement C2: 0.31 ng/mL
- Complement C4b: 0.36 ng/mL
- Complement C5: 1.040 ng/mL
- Complement C5a: 0.0051 ng/mL
- Complement C9: 10.401 ng/mL
- Adipsin (Complement Factor D): 0.026 ng/mL
- Mannose-Binding Lectin (MBL): 0.040 ng/mL
- Complement Factor I: 0.22 ng/mL
|
| Standard Curve Range |
- Complement C2: 1.37 – 1,000 ng/mL
- Complement C4b: 1.37 – 1,000 ng/mL
- Complement C5: 2.74- 2,000 ng/mL
- Complement C5a: 4.12 – 30,000 pg/mL
- Complement C9: 41.2– 30,000 ng/mL
- Adipson/ Complement Factor D: 0.069 – 50 ng/mL
- Mannose-Binding Lectin (MBL): 0.137 – 100 ng/mL
- Complement Factor I: 0.69 - 500 ng/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96 well plate |
