| Description | |
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| Catalogue Number | FTAV00003 |
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| Description | Fast Trap Adenovirus Purification and Concentration Kit |
| Overview | The Fast-Trap Adenovirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification of ≥ 3 mL of adenovirus serotype 5, up to a capacity of approximately 1x1013 total adenovirus particles. The purification kit protocol calls for releasing the virus from the infected cells using multiple freeze-thaw cycles, then collecting the crude adenovirus. Most of the large cellular debris is removed by centrifugation, leaving the viable virus particles in the supernatant. The supernatant is further clarified by passing it through a Steriflip-HV 0.45 μm filter. After adding a binding buffer, the virus solution is passed over a treated filter that adsorbs the virus particles, allowing the smaller cellular debris and media to pass through the filter. Following a wash to remove any weakly bound proteins, the virus is eluted off the filter with an elution buffer. Finally, the virus can be concentrated and exchanged into the desired buffer using an Amicon® Ultra centrifugal filter unit. For laboratory use only; not for diagnostic or in vivo use. |
| Background Information | millipore’s Fast-Trap Adenovirus and Lentivirus Purification and Concentration Kits provide a fast, safe, and easy alternative for viral purification. The kits contain the necessary components to accommodate the entire virus purification workflow. The purification results in high recovery of viable viral particles with good purity. Fast-Trap Advantages - Saves time: protocol can be completed in as little as two hours - Higher yield: recover up to 70% of viral particles - Dependable: simple protocol ensures quality results - Easy: Steriflip filters eliminate messy purification steps and expensive equipment - Safe: close vacuum device eliminate potential spills Faster: In one experiment, we tested millipore’s Fast-Trap kit with other common methods to determine which methods gave the best recoveries in the least time. The Fast-Trap Kit was faster than the other methods while giving similar recovery. |
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| Quality Level | MQ200 |
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| Storage Conditions | The Fast-Trap Adenovirus Purification and Concentration Kit should be stored at room temperature. Warnings and Precautions: WARNING: This kit permits quick purification of adenovirus, an infectious agent which should be handled under BSL2 safety precautions. Wear hand, eye, face and body personal protective equipment (PPE) when using this kit. millipore Corporation takes no responsibility for improper use of this kit. WARNING:All virus purification steps (except water bath incubation and centrifugation) should be conducted in a biosafety cabinet. For research use only. Not for use in diagnostic or human clinical procedures. Variation in the amount of adenovirus purified with this kit may be due in part to infection success and cell conditions. |
| Packaging Information | |
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| Material Size | 3 purifications and concentrations |
| Material Package | 3 purifications and concentrations |
