| Description
|
| Overview | The SpinPrep™ PCR Clean-Up Kit is designed
for the rapid purification of DNA amplified
in PCR. The 10-minute procedure involves
addition of a binding buffer followed by
adsorption of the DNA to a silica membrane in
a spin column format. Following a wash step,
the DNA is eluted in low-salt buffer. This kit
removes DNA polymerases, dNTPs, salts, and
> 99% of primers so that they do not interfere
with downstream applications such as cloning,
sequencing, or labeling. PCR products from
100 bp to > 12,000 bp can be cleaned up, with
recoveries of amplified DNA in the range of
60-90% under standard conditions.
| |
|
| Column binding capacity: |
up to 6 µg |
| PCR volume: |
100 µl/rxn |
| Typical recovery: |
60–90% |
| Size range: |
100 bp to > 12,000 bp |
| Time required: |
< 10 min |
 |
| Catalogue Number | 70976 |
| Brand Family |
Novagen®
|
| Product Information
|
| Components |
|
| Storage and Shipping Information
|
| Ship Code |
Shipped at Ambient Temperature or with Blue Ice
|
| Toxicity |
Multiple Toxicity Values, refer to MSDS
|
| Storage |
+15°C to +30°C
|
| Do not freeze |
Ok to freeze
|
