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密理博,46-610MAG,MILLIPLEX MAP Phospho p38α/SAPK2 (Thr180/Tyr182) Magnetic Bead MAPmate - Cell Signaling Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| p38α/SAPK2 (Thr180/Tyr182)
| H, M, R
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | 46-610MAG |
| Trade Name |
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| Description | MILLIPLEX MAP Phospho p38α/SAPK2 (Thr180/Tyr182) Magnetic Bead MAPmate™ - Cell Signaling Multiplex Assay |
| Overview | The MILLIPLEX® MAP Phospho p38α/SAPK2 (Thr180/Tyr182) Magnetic Bead MAPmate™ pair is used in conjunction with the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602MAG) to detect the presence of Phospho p38α/SAPK2 (Thr180/Tyr182) in cell lysates using the Luminex 100™ IS, 200™, HTS, FLEXMAP 3D®, MAGPIX® system. Each MAPmate™ pair is ordered individually and may be combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common set of reagents needed for performing MAPmate™ assays. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX® MAP HeLa Cell Lysate: Unstimulated included in the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit may be utilized as an unstimulated control for this target, while the HeLa Cell Lysate: HS/Ars included with this MAPmate™ may be utilized as a stimulated control.
Important note: For a detailed protocol on Cell Signaling Detection Procedures please see the instruction booklet for the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602MAG). |
| Materials Required but Not Delivered | • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602) |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Single Plex |
| Panel Type | Cell Signaling |
| Quality Level | MQ200
|
| Applications
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| Application | Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting. |
| Key Applications |
|
| Application Notes | • An overnight (4°C) incubation is recommended for best results
• This assay requires 25 µL diluted cell lysate per well
• Please consult the buffer compatibility chart or protocol for appropriate buffer choice
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg) |
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
- p38α/SAPK2 (Thr180/Tyr182)
|
| Entrez Gene Number |
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| UniProt Number |
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| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Storage and Shipping Information
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C |
| Packaging Information
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| Material Size | 96-well plate |
