millipore,密理博,46-604MAG,MILLIPLEX MAP Caspase 3 (Active) Magnetic Bead MAPmate - Cell Signaling Single Plex
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
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| Caspase 3 (Active) | H, M | Mplex | Luminex xMAP |
| Description | |
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| Catalogue Number | 46-604MAG |
| Replaces | MILLIPLEX Late Apoptosis-- 3 Plex (Cat. No. 48-670MAG) |
| Trade Name |
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| Description | MILLIPLEX MAP Caspase 3 (Active) Magnetic Bead MAPmate - Cell Signaling Single Plex |
| Background Information | Caspase 3 (Active) • A member of the Cysteine-aspartic acid protease (Caspase) family • Caspases exist as inactive proenzymes that undergo proteolytic processing at aspartic residues to produce two subunits that dimerize to form the active enzyme • Associated with brain development, dysfunction and possibly stem cell differentiation |
| Materials Required but Not Delivered | • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602) |
| Product Information | |
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| Detection method | Luminex xMAP |
| Configuration | Single Plex |
| Panel Type | Cell Signaling |
| Quality Level | MQ200 |
| Applications | |
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| Application | MAPmate single plexes allow you to design your own cell signaling panel. The analyte available for this kit is Caspase 3 (Active). |
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| Application Notes | • An overnight (4°C) incubation is recommended for best results • These assays require 25 µL diluted cell lysate per well • Please consult the buffer compatibility chart or protocol for appropriate buffer choice • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg |
| Biological Information | |
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| Species Reactivity |
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| Analytes Available |
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| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. SIZE: 277 amino acids; 31608 Da SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C |
| Packaging Information | |
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| Material Size | 96-well plate |
