millipore,密理博,46-602MAG,MILLIPLEX MAP Phospho ERK/MAPK 1/2 (Thr185/Tyr187) Magnetic Bead MAPmate - Cell Signaling Single Plex
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|---|---|---|
| Erk/MAPK 1/2 (Thr185/Tyr187) | H, M | Mplex | Luminex xMAP |
| Description | |
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| Catalogue Number | 46-602MAG |
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| Description | MILLIPLEX MAP Phospho ERK/MAPK 1/2 (Thr185/Tyr187) Magnetic Bead MAPmate - Cell Signaling Single Plex |
| Materials Required but Not Delivered | • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602) |
| Product Information | |
|---|---|
| Detection method | Luminex xMAP |
| Configuration | Single Plex |
| Panel Type | Cell Signaling |
| Quality Level | MQ200 |
| Applications | |
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| Application | MAPmate single plexes allow you to design your own cell signaling panel. The analyte available for this kit is Erk/MAPK 1/2 (Thr185/Tyr187). |
| Key Applications |
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| Application Notes | • An overnight (4°C) incubation is recommended for best results • This assay requires 25 µL diluted cell lysate per well • Please consult the buffer compatibility chart or protocol for appropriate buffer choice • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg) |
| Biological Information | |
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| Species Reactivity |
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| Analytes Available |
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| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). COFACTOR: Magnesium (By similarity). SIZE: 360 amino acids; 41390 Da SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4. DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
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| Packaging Information | |
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| Material Size | 96-well plate |
| Material Package | 96-well Plate |
