重要规格表
| Description
|
| Overview | Native DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units |
| Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units. |
| Catalogue Number | 260913 |
| Brand Family |
Calbiochem®
|
| Synonyms | Deoxyribonuclease I |
| References
|
| References | Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun. 269, 481.
Jones, S.J., et al. 1996. J. Mol. Biol. 264, 1154. |
| Product Information
|
| CAS number | 9003-98-9 |
| Activity | ≥60,000 Dornase units/mg dry weight. |
| Unit of Definition | One Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C. |
| EC number | 3.1.21.1 |
| Form | Lyophilized |
| Hygroscopic |
Hygroscopic
|
| Safety Information according to GHS
|
| RTECS | RF0750000 |
| Storage and Shipping Information
|
| Ship Code |
Ambient Temperature Only
|
| Toxicity |
Standard Handling
|
| Storage |
-20°C
|
| Protect from Moisture |
Protect from moisture
|
| Hygroscopic |
Hygroscopic
|
| Do not freeze |
Ok to freeze
|
| Special Instructions | Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. |
