millipore,
密理博,03-0108-00,SMC Mouse TNF-alpha (TNF-α) Immunoassay KitIMMUNOASSAY KIT
重要规格表
| Species Reactivity | Key Applications |
|---|
| M
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0108-00 |
| Trade Name |
|
| Description | SMC™ Mouse TNF-alpha (TNF-α) Immunoassay Kit
IMMUNOASSAY KIT |
| Overview | The SMC™ Mouse TNF-α Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine with cytotoxic activity that is primarily produced by macrophages. TNF-α is involved in systemic inflammation and is a member of the group of cytokines that stimulate the acute phase reaction. When administered to animals or humans it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation and acute phase responses similar to those seen during acute infections and shock states. By promoting the inflammatory response, TNF-α is associated with many autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn’s disease, psoriasis and refractory asthma. TNF-α also plays a role in cancer and regulates apoptotic cell death, cellular proliferation, differentiation, tumorigenesis and viral replication. |
| Product Information
|
| Components |
- Mouse TNFα Coated Beads
- Standard Diluent
- Mouse TNFα Detection Antibody
- Assay Buffer
- Mouse TNFα Standard
- SMC™ Mouse TNFα Immunoassay Kit Instructions
- 10X Wash Buffer
- Elution Buffer B
- Buffer D
|
| Precision, % |
- Low-end CV% Range: 0 - 17%
- Low-end CV% Average: 8%
|
| Quality Level | MQ200
|
| Applications
|
| Application | The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Mouse TNF-a Immunoassay Kit unparalleled performance in the quantification of TNF-α in mouse serum. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Biological Information
|
| Species Reactivity |
|
| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
|
| Physicochemical Information
|
| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96 well assay |
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