millipore,
密理博,03-0099-01,SMC Total-AKT1 Immunoassay KitIMMUNOASSAY KIT
重要规格表
| Species Reactivity | Key Applications |
|---|
| H, R, M
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0099-01 |
| Trade Name |
|
| Description | SMC™ Total-AKT1 Immunoassay Kit
IMMUNOASSAY KIT |
| Overview | The SMC™ Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | The serine-threonine protein kinase AKT family is the primary downstream mediator of the phosphatidylinositol-3-kinase (PI3K) signaling pathway. Members of the family (AKT1, AKT2, and AKT3) require two separate kinases for complete activation. AKT1 phosphorylates mTOR, whose downstream targets include other kinases, cell cycle regulators and transcription factors that are essential for many cell proliferation and survival processes. AKT1 also up-regulates matrix metalloproteinases and is thus implicated in tumor progression. AKT1 and other members of the AKT family have been an important focus in therapeutic development with their broad effects on the PI3K pathway. |
| Product Information
|
| Components |
- TOTAL AKT1 Coated Beads
- Sample Diluent
- TOTAL AKT1 Detection Antibody
- Assay Buffer
- AKT1 Standard
- AKT1 Detection Antibody Buffer
- 10X Wash Buffer
- Elution Buffer B
- Buffer D
- SMC™ TOTAL AKT1 Immunoassay Kit Instructions
|
| Precision, % |
- Low-end CV% Average: 10%
- Low-end CV% Range: 2 - 15%
|
| Quality Level | MQ200
|
| Applications
|
| Application | The sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Total AKT-1 Immunoassay Kit unparalleled performance in the quantification of Total AKT-1 in cell lysates. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Biological Information
|
| Species Reactivity |
|
| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
|
| Physicochemical Information
|
| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96 well assay |
[03-0092-00_Human%20cTnI%20(1)-ALL].jpg "millipore,密理博,03-0092-00,SMC Human Cardiac Troponin I (cTnI)IMMUNOASSAY KIT")
