millipore,密理博,WB59,Western-Re-Probe Reagent

Description
Overview	Designed to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.
Overview	A special formulation designed to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity.
Catalogue Number	WB59
Brand Family	Calbiochem®

Applications
Key Applications	Immunoblotting (Western Blotting)
Application Notes	Immunoblotting (see comments)
Application Comments	Sufficient volume to strip 25-30 standard blots. ADVANTAGES -Immunoblotting analyzed repeatedly with multiple probes -Saving on hard-to-get reagents and samples -Correction and re-analysis of unsatisfactory Western Blots -Saving time spent in gel re-running and transfer processes PROTOCOL 1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water. 2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking. 3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween^®-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer. 4- Block the membrane and perform immunodetection. Western-Re-Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing. PROTOCOL SUMMARY 1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shaking 2. Wash the membrane 3 times in PBST or TBST buffer 3. Block membrane and perform immunodetection