millipore,
密理博,TGFBMAG-64K-03,MILLIPLEX MAP TGFß Magnetic Bead 3 Plex Kit - Immunology Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| TGF-β1, TGF-β2, TGF-β3
| H, M, R, NhP, Ca, Fe, Po, Eq
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | TGFBMAG-64K-03 |
| Trade Name |
|
| Description | MILLIPLEX MAP TGFß Magnetic Bead 3 Plex Kit - Immunology Multiplex Assay |
| Background Information | The transforming growth factor beta (TGFβ) system, a superfamily of cytokines as well as signaling pathways, is highly conserved throughout the animal kingdom. TGFβ functions in immune cell development, angiogenesis, wound healing and embryonic development, bone development and plays a critical role in immune response, heart disease, and cancer. In its normal state TGFβ is one of the few classes of proteins able to inhibit cell growth by halting mitosis at the G1 state, inducing cell differentiation or apoptosis. However, during oncogenesis mutations in the TGFβ signaling pathway result in tumor cell resistance to the effects of normally functioning TGFβ, causing proliferation without regulation. Initial research suggests that VE-cadherin may enhance the mutated TGFβ signaling pathway. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Premixed |
| Precision, % |
- Inter-assay: < 10
- Intra-assay: < 10
|
| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200
|
| Applications
|
| Application | This MILLIPLEX MAP TGFß (Transforming Growth Factor Beta) - 3 Plex is an Immunology Multiplex Assay is used for the simultaneous quantification of TGFß1, TGFß2 and TGFß3 in the following species: Mouse/Human/Rat/Pig/Horse/Dog/Cat/Non-Human Primate. |
| Key Applications |
|
| Application Notes | • This is a 2-hour or overnight incubation assay.
• This assay requires 25 μL of a 1:30 dilution after treatment of serum and plasma samples.
• In addition to human, TGFβ1 was detected in acid-activated serum of every species tested which included horse, rabbit, mouse, rat, mini pig, canine, hamster, guinea pig, rhesus monkey and cynomolgous monkey. In addition to human, TGFβ2 was detected in the acid-activated serum of the following: horse, rabbit, mouse, rat, mini pig, canine, hamster, guinea pig, rhesus monkey and cynomolgous monkey. In addition to human, TGFβ3 was detected in mouse, rat, mini pig, hamster, guinea pig, rhesus monkey and cynomolgous monkey samples.
However, the exact amount of cross reactivity has not been determined. |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
- Human
- Mouse
- Rat
- Non-human Primate
- Canine
- Feline
- Pig
- Horse
|
| Analytes Available |
|
| Assay Panel | Premixed beads for your convenience. |
| Accuracy |
|
| Physicochemical Information
|
| Sensitivity |
|
| Standard Curve Range |
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
