millipore,
密理博,MTH17MAG-47K,MILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Immunology Multiplex Assay
| Description
|
| Catalogue Number | MTH17MAG-47K |
| Trade Name |
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| Description | MILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Immunology Multiplex Assay |
| Overview | The MILLIPLEX MAP Mouse Th17 Magnetic Bead panel enables you to focus on the therapeutic potential of the modulation of CD4 T-helper cell cytokines. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity. |
| Background Information | CD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFN, secrete IFN that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.
Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn's disease and autoimmune uveitis. In addition Th17 cells play an important role in tumor development, progression and metastasis, potentially in both promoting and inhibiting tumor growth. |
| Product Information
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| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: < 15
- Intra-assay:< 10
|
| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200
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| Applications
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| Application | Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples. |
| Key Applications |
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| Application Notes | • This is a two-hour or overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Th17 panel analytes in serum, plasma or tissue/cell lysate and culture supernatant |
| Biological Information
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| Species Reactivity |
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| Analytes Available |
- CD40 Ligand
- GM-CSF
- IFN-γ
- IL-1β
- IL-2
- IL-4
- IL-5
- IL-6
- IL-10
- IL-12 (p70)
- IL-13
- IL-15
- IL-17A
- IL-17E/IL-25
- IL-17F
- IL-21
- IL-22
- IL-23
- IL-27
- IL-28B
- IL-31
- IL-33
- MIP-3α/CCL20
- TNF-α
- TNFβ
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| Cross Reactivity | There was no or minimum cross-reactivity between the antibodies and any other analytes in the panel (see details in assay protocol). |
| Physicochemical Information
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| Sensitivity |
- Refer to kit protocol for sensitivities of individual biomarkers.
|
| Linearity of Dilution | 1:4 111.0% 1:8 113.1% |
| Standard Curve Range |
- CD40L IL-31 & MIP-3α: 49 – 50,000 pg/mL
- GM-CSF & IL-15: 34 – 35,000 pg/mL
- IFNγ & IL-6: 7.8 – 8,000 pg/mL
- IL-1β: 15 – 15,000 pg/mL
- IL-2: 6.9 – 6,000 pg/mL
- IL-4: 1.5 – 1,500 pg/mL
- IL-5: 4.9 – 5,000 pg/mL
- IL-10, IL-12(p70), & IL-21:20 – 20,000 pg/mL
- IL-13 & IL-17A: 39 – 40,000 pg/mL
- IL-17E/ IL-25: 586 – 600,000 pg/mL
- IL-17F: 10 – 10,000 pg/mL
- IL-22: 2.4 – 2,500 pg/mL
- IL-23: 342 -350,000 pg/mL
- IL-27: 879 – 900,000 pg/mL
- IL-28B: 127 – 130,000 pg/mL
- IL-33: 78 – 80,000 pg/mL
- TNFβ:488 – 500,000 pg/mL
- TNFα: 3.4 – 3,500 pg/mL
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| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Storage and Shipping Information
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
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| Material Size | 96-well plate |
