重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|---|---|---|
| BDNF, Erythropoietin (EPO), FGF-21, Follistatin-like Protein 1 (FSTL1), Fractalkine/CX3CL1, IL-6, IL-15, Irisin, LIF, Myostatin/GDF8, Oncostatin M (OSM), Osteocrin/Musclin, Osteonectin (SPARC) | M | Mplex | Luminex xMAP |
| Description | |
|---|---|
| Catalogue Number | MMYOMAG-74K |
| Trade Name |
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| Description | MILLIPLEX MAP Mouse Myokine Magnetic Bead Panel |
| Overview | MILLIPLEX® MAP Mouse Myokine Magnetic Bead Panel is a 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture samples: • Brain-derived Neurotrophic Factor (BDNF) • Erythropoeitin (EPO)* • Fibroblast Growth Factor 21 (FGF21) • Follistatin-Like Protein 1 (FSTL-1) • Fractalkine (FKN / CX3CL1) • IL-6 • IL-15 • Irisin • Leukemia Inhibitory Factor (LIF) • Myostatin (MSTN / GDF8) • Oncostatin M (OSM) • Osteocrin/Musclin (OSTN) • Osteonectin (SPARC) *Only available for cells culture samples. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. Each MILLIPLEX® MAP panel and kit includes: • Quality controls (QCs) provided to qualify assay performance • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency • Optimized serum matrix to mimic native analyte environment • Detection antibody cocktails designed to yield consistent analyte profiles within panel EMD millipore’s MILLIPLEX® MAP Mouse Myokine Magnetic Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation. |
| Background Information | Skeletal muscle is actively involved in the synthesis and secretion of many proteins collectively termed “myokines.” Muscle contraction during physical activity is an important activator for the release of myokines, which act in an autocrine and/or paracrine manner to regulate skeletal muscle growth. Myokines also act as endocrine hormones which mediate inter-organ crosstalk. Myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue as well as for profound changes in immune and neuron systems. Various genetically engineered mouse strains and disease models are now serving as exceptional research tools for human myokine study. |
| Product Information | |
|---|---|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
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| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200 |
| Applications | |
|---|---|
| Application | Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple myokine and cytokine biomarkers in mouse serum and plasma samples. |
| Key Applications |
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| Application Notes | • This assay uses 25 µL of a 1:1 dilution of serum or plasma per well, or 25 µl neat or appropriately diluted tissue culture supernatant per well. • For optimal sensitivity, use an overnight (16-18 hour) incubation. A two hour incubation can also be used (though sensitivity may be lower). |
| Biological Information | |
|---|---|
| Species Reactivity |
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| Analytes Available |
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| Cross Reactivity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Accuracy |
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| Physicochemical Information | |
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| Standard Curve Range |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information | |
|---|---|
| Material Size | 96-well plate |
| Material Package | 96 well plate |
