millipore,
密理博,HSP2MAG-63K,MILLIPLEX MAP Human Sepsis Magnetic Bead Panel 2 - Immune Response Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| Granzyme B, HSP70, IL-1α, IL-8, MIP-1α, MIP-1β, MMP-8
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HSP2MAG-63K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Sepsis Magnetic Bead Panel 2 - Immune Response Multiplex Assay |
| Background Information | Sepsis or SIRS (systemic inflammatory response syndrome) results from infection, whether bacterial, viral, fungal or parasitic. According to the CDC, sepsis is the second leading cause of death in non-coronary ICU patients in the U.S. The pathogenesis of sepsis is very complex. Both an innate and acquired immune response to infection interrupts homeostasis with a shift of cytokine expression that increases immunosuppression. In addition migration of the leukocytes in the inflamed tissues secretes a tissue factor leading to the formation of thrombin, also triggering the release of chemokines and adhesion molecules through endothelial cells. Severe sepsis occurs when hypotension or hypoperfusion to one or more organs leads to organ dysfunction, which, in turn, can cause septic shock, multiple organ dysfunction syndrome (MODS) and death. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: < 15
- Intra-assay: < 10
|
| Panel Type | MAGNETIC Immune Response |
| Quality Level | MQ200
|
| Applications
|
| Application | Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | • This is a 2-hour incubation assay.
• This assay requires 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well. |
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
- Granzyme B
- HSP70
- IL-1α
- IL-8
- MIP-1α
- MIP-1β
- MMP-8
|
| Cross Reactivity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Accuracy |
- Granzyme B 95%
- HSP70 92%
- IL-1α 97%
- IL-8 98%
- MIP-1α 92%
- MIP-1β 87%
- MMP-8 92%
|
| Physicochemical Information
|
| Sensitivity |
- Refer to kit protocol for individual sensitivities.
|
| Standard Curve Range |
- Granzyme B: 2 - 2,000 pg/mL
- HSP70: 2,930 - 3,000,000 pg/mL
- IL-1α and MIP-1β: 10 - 10,000 pg/mL
- IL-8 and MIP-1β: 5 - 5,000 pg/mL
- MMP-8: 195 - 200,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96-well plate |
