millipore,密理博,HCYP2MAG-62K,MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay
| Description | |
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| Catalogue Number | HCYP2MAG-62K |
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| Description | MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay |
| Overview | To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include: • More flexible plate and plate washer options • Improved performance with turbid serum/plasma samples • Assay results equivalent to non-magnetic beads • Automated magnetic washing avoids many problems associated with vacuum filtration washing Therefore, the MILLIPLEX® MAP Human Cytokine / Chemokine Panel II enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity. References: PLoS One. 2016 Feb 26;11(2):e0149411. doi: 10.1371/journal.pone.0149411. eCollection 2016. B Cell, Th17, and Neutrophil Related Cerebrospinal Fluid Cytokine/Chemokines Are Elevated in MOG Antibody Associated Demyelination. Kothur K, Wienholt L, Tantsis EM, Earl J, Bandodkar S, Prelog K, Tea F, Ramanathan S, Brilot F, Dale RC |
| Background Information | “Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer. |
| Product Information | |
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| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
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| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200 |
| Applications | |
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| Application | Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples. |
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| Application Notes | • This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended. • This assay requires 25 µl of neat plasma or serum, or 25 µL cell culture supernatant per well. • This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples. |
| Biological Information | |
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| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies and any of the other analytes in this panel. |
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| Linearity of Dilution | Contact Technical Service for this value |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information | |
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| Material Size | 96-well plate |
| Material Package | 96 well plate |
