millipore,
密理博,CKT2MAG-97K,MILLIPLEX MAP Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| Albumin, β-2-Microglobulin, Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)
| Ca
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | CKT2MAG-97K |
| Trade Name |
|
| Description | MILLIPLEX® MAP Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay |
| Overview | The MILLIPLEX® MAP Canine Kidney Toxicity Panel 2 contains all the components necessary to measure the following four biomarkers in any combination using Luminex® xMAP® technology: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4). The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 urine samples in duplicate. |
| Background Information | As one of the leading causes of drug candidate attrition, toxicity research is receiving more attention, especially in the search of sensitive, organ-specific toxicity biomarkers.
In pharmaceutical and chemical industries, renal function is routinely assessed during toxicity evaluations. The importance of the kidney as a central detoxification organ leads to a high exposure of renal tissue to drugs, reactive metabolites, and environmental compounds. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. For improved, non-invasive detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species.
EMD millipore provides a valuable research assay to investigate multiple biomarkers of kidney injury in canine urine samples using the Luminex xMAP® instrument platform. The MILLIPLEX® MAP Canine Kidney Toxicity Panel 2 offers simultaneous analysis of any combination of the following analytes: Albumin*, β-2-microglobulin (B2M)*, Trefoil Factor 3 (TFF-3)*, Retinol Binding Protein 4 (RBP4)*.
*Urinary albumin, B2M, TFF-3 and RBP4 are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. |
| Product Information
|
| Components |
- Canine Kidney Toxicity Panel 2 Standard, 1 vial, lyophilized
- Canine Kidney Toxicity Panel 2 Control 1, 1 vial, lyophilized
- Canine Kidney Toxicity Panel 2 Control 2, 1 vial, lyophilized
- Assay Buffer, 2 bottles, 30 mL
- Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL
- Canine Kidney Toxicity Panel 2 Detection Antibodies, 1 bottle, 5.5 mL
- Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
- Mixing Bottle, 1 bottle
- Sample Plate, 96 well format, 1 plate
- 2 Foil Plate Sealers
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: <15
- Intra-assay: <10
|
| Panel Type | MAGNETIC Toxicity |
| Quality Level | MQ200
|
| Applications
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| Application | The analytes available for this multiplex kit are: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4) |
| Key Applications |
|
| Application Notes | • Customers should determine the optimal dilution factor for their samples. Our recommendations are based on urine samples from normal subjects.
• This assay requires 25 µL of 1:500 diluted urine sample per well in kit Assay Buffer (please dilute per protocol instructions).
• This is a 2 hour assay. |
| Biological Information
|
| Specificity | Approximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel. |
| Species Reactivity |
|
| Analytes Available |
- Albumin
- β-2-Microglobulin
- Trefoil Factor 3 (TFF-3)
- Retinol Binding Protein 4 (RBP4)
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | Approximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel. |
| Accuracy |
|
| Physicochemical Information
|
| Sensitivity |
- Albumin: 12.48 ng/mL (MinDC +2SD)
- All other analytes: 0.016 - 0.026 ng/mL (MinDC +2SD)
- See kit protocol for individual analyte sensitivities.
|
| Linearity of Dilution | Contact technical support for this information. |
| Standard Curve Range |
- Albumin: 12.21 – 50,000 ng/mL
- B2M: 0.02 – 100 ng/mL
- TFF-3: 0.02 – 100 ng/mL
- RBP4: 0.01 – 50 ng/mL
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| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Storage and Shipping Information
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
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| Material Size | 96-well plate |
