| Description
|
| Overview | The BugBuster® GST•Bind™ Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag™ fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.
 |
| Catalogue Number | 70794 |
| Brand Family |
Novagen®
|
| Product Information
|
| • | 2 x 100 ml | BugBuster Protein Extraction Reagent
|
| • | 10,000 U | Benzonase Nuclease, purity > 90%
|
| • | 10 ml | GST•Bind Resin
|
| • | pkg/4 | Chromatography Columns
|
| • | 2 x 100 ml | 10X GST Bind/Wash Buffer
|
| • | 40 ml | 10X Glutathione Reconstitution Buffer
|
| • | 1 g | Glutathione, Reduced |
| Safety Information
|
| R Phrase | R: 36/38
Irritating to eyes and skin. |
| S Phrase | S: 26
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| Storage and Shipping Information
|
| Ship Code |
Blue Ice Only
|
| Toxicity |
Multiple Toxicity Values, refer to MSDS
|
| Storage |
Multiple storage requirements
|
| Do not freeze |
Ok to freeze
|
