| Description
|
| Overview | Benzonase® Nuclease is a genetically
engineered endonuclease from Serratia
marcescens. It degrades all forms of DNA and
RNA (single stranded, double stranded, linear
and circular) while having no proteolytic
activity. It is effective over a wide range of
conditions and possesses an exceptionally
high specific activity. The enzyme completely
digests nucleic acids to 5′-monophosphate
terminated oligonucleotides 2 to 5 bases in
length (below the hybridization limit), which
is ideal for removal of nucleic acids from
recombinant proteins, enabling compliance
with FDA guidelines for nucleic acid
contamination. The ability of Benzonase to
rapidly hydrolyze nucleic acids makes the
enzyme an excellent choice for viscosity
reduction to reduce processing time and
increase yields of protein. For example, the
enzyme is compatible with BugBuster® and
PopCulture® Protein Extraction Reagents
and can therefore be added along with these
reagents to eliminate viscosity and remove
nucleic acids from E. coli extracts.
The enzyme consists of two subunits of
30 kDa each. It is functional between
pH 6 and 10 and from 0-42°C and requires
1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF
(1 mM), EDTA (1 mM) and urea (relative
activity depends on specific conditions).
Activity is inhibited by > 150 mM monovalent
cations, > 100 mM phosphate, > 100 mM
ammonium sulfate, or > 100 mM guanidine HCl.
Benzonase Nuclease is available in
ultrapure (> 99% by SDS-PAGE) and pure
(> 90%) grades at a standard concentration
of 25-29 U/µl and at a high concentration (HC)
of 250 U/µl. Both preparations are free of
detectable protease and have specific activity
> 1 × 106 U/mg protein. The > 99% purity
grade is tested for endotoxins and contains
< 0.25 EU/1000 units. The product is supplied
in 50% glycerol.
Store at -20°C.
Total endotoxin: < 0.25 EU/1,000 units.
Purity: > 90% by SDS-PAGE
|
| Catalogue Number | 70746 |
| Brand Family |
Novagen®
|
| Product Information
|
| Unit of Definition | One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA. |
| Quality Level | MQ300
|
| Biological Information
|
| Purity | Purity: > 90% by SDS-PAGE. |
| Storage and Shipping Information
|
| Ship Code |
Blue Ice Only
|
| Toxicity |
Standard Handling
|
| Storage |
-20°C
|
| Do not freeze |
Ok to freeze
|
