| Description
|
| Overview | RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation. For applications requiring the use of DNase I
for random double-stranded cleavage of DNA, try DNase I, ds Qualified. |
| Catalogue Number | 69182 |
| Brand Family |
Novagen®
|
| Synonyms | RNase-free DNase I |
| Product Information
|
| Unit of Definition | One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme. |
| Storage and Shipping Information
|
| Ship Code |
Shipped with Blue Ice or with Dry Ice
|
| Toxicity |
Standard Handling
|
| Storage |
-20°C
|
| Avoid freeze/thaw |
Avoid freeze/thaw
|
| Do not freeze |
Ok to freeze
|
