重要规格表
| Format | Detection Methods |
|---|---|
| Liquid, Antibody Diluent | Chemiluminescence, colorimetric, or fluorescence |
| Description | |
|---|---|
| Overview | SignalBoost™ Immunoreaction Enhancer Kit is designed to enhance the desired signal in assays such as immunoblotting, dot blotting, and ELISA. This kit significantly improves sensitivity and discrimination when assessing protein targets of low immunoreactivity or low expression levels. Enhancement of signal is especially marked for low affinity antibodies.
It replaces conventional antibody diluents with Solution 1, which is used to dilute primary antibodies and Solution 2, which is used to dilute secondary antibodies. No additional steps, materials, or increase of assay time is required.
• Increases signal: minimizes background resulting in high signal to noise ratios • Versatile: can be used with nitrocellulose or PVDF membranes and is compatible with chemiluminescent or colorimetric detection systems • Convenient: ready-to-use format, no dilution necessary |
| Catalogue Number | 407207 |
| Brand Family | Calbiochem® |
| Application Data |  Detection of protein kinase C by immunoblotting. Samples: whole cell lysate from K562 cells. Immunoblotting conditions: nitrocellulose membrane blocked overnight with 5% milk in PBST; all washes were performed with PBST. Primary antibody: Anti-PKC (Ab-2) Mouse mAb (MC5) (Cat. No. OP74) diluted 1:1000 in 5% milk/PBST (lane A) or in Solution 1 (lane B) (incubation for 1 h ). Secondary antibody: Anti-Mouse IgG diluted 1:10,000 in 5% milk/PBST (lane A) or Solution 2 (lane B). Detection: chemiluminescence (30 s exposure).  ELISA results generated with and without dilution of antigen and detection antibody using SignalBoost™ reagent. |
| Materials Required but Not Delivered | • Any equipment necessary for performing the desired immunoassay • Primary antibody • Secondary antibody (labeled with HRP, AP, etc.) specific to the primary antibody |
| Product Information | |
|---|---|
| Detection method | Chemiluminescence, colorimetric, or fluorescence |
| Form | 20 Miniblots or 1600 cm² |
| Format | Liquid, Antibody Diluent |
| Kit contains | Solution 1 for Primary Antibodies, Solution 2 for Secondary Antibodies, and a user protocol. |
| Product Usage Statements | |
|---|---|
| Intended use | SignalBoost™ Immunoreaction Enhancer Solution is a reactive reagent for use in immunoassays, and was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as immunoblotting and ELISA (enzyme-linked immunosorbent assay). It can be used in a range of immunoassays. |
| Storage and Shipping Information | |
|---|---|
| Ship Code | Blue Ice Only |
| Toxicity | Standard Handling |
| Storage | +2°C to +8°C |
| Storage Conditions | Upon receipt, store the entire contents of the kit at 4°C. Protect from light. Following initial use, this product is stable for up to 1 year at 4°C. |
| Protect from Light | Protect from light |
| Do not freeze | Ok to freeze |
| Supplemental Information | |
|---|---|
| Kit contains | Solution 1 for Primary Antibodies, Solution 2 for Secondary Antibodies, and a user protocol. |
