重要规格表
| Description | |
|---|---|
| Overview | Designed to minimize waste of antibody in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. When applied in a circle around the specimen, forms a hydrophobic barrier, retaining antibody. Sufficient for 500-800 individual applications. Not available for sale in Japan. |
| Catalogue Number | 402176 |
| Brand Family | Calbiochem® |
| Product Information | |
|---|---|
| Declaration | Not available for sale in Japan. |
| Applications | |
|---|---|
| Application Notes | Frozen Sections Immunostaining Barrier Paraffin Sections |
| Application Comments | To start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip. Paraffin Section Method: Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed. Frozen Section Method: Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water. |
| Storage and Shipping Information | |
|---|---|
| Ship Code | Ambient Temperature Only |
| Toxicity | Standard Handling |
| Storage | +15°C to +30°C |
| Do not freeze | Ok to freeze |
| Special Instructions | Keep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications. |
