millipore,
密理博,03-0025-06,SMC Human Total Glucagon-Like Peptide-1 (Total GLP-1) IMMUNOASSAY KIT
重要规格表
| Analytes Available | Species Reactivity | Key Applications |
|---|
| GLP-1 (Total)
| H, M, R, Ca
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0025-06 |
| Trade Name |
|
| Description | SMC™ Human Total Glucagon-Like Peptide-1 (Total GLP-1)
IMMUNOASSAY KIT |
| Overview | The SMC™ Total GLP-1 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Glucagon-Like Peptide-1 (GLP-1) is a member of the incretin family of hormones, which are released by the gut into the bloodstream in response to food. Post-translational cleavage of the peptide produces active GLP-1, which is then rapidly degraded by protease dipeptidyl peptidase-4 (DPP-4). When blood glucose level is high, active GLP-1 enhances insulin secretion and also suppresses the release of glucagon. In addition, GLP-1 stimulates the brain to trigger feelings of satiety and regulates the rate of gastric emptying. Compared to healthy controls, some patients with type-2 diabetes show a modest decrease in GLP-1 release. Therapeutic GLP-1 analogs such as exenatide, or DPP inhibitors like sitagliptin, are promising strategies for treating type-2 diabetes. |
| Product Information
|
| Components |
- Total GLP-1 Coated Beads Vial 1
- Total GLP-1 Coated Beads Vial 2
- Total GLP-1 Standard Diluent
- GLP-1 Assay Buffer
- Total GLP-1 Standard
- Total GLP-1 Detection Antibody
- SMC™ Total GLP-1 Immunoassay Kit Instructions
- 10X Wash Buffer
- Elution Buffer B
- Buffer D
|
| Precision, % |
- Low-end CV% Range: 5 - 12%
- Low-end CV% Average: 9%
|
| Quality Level | MQ200
|
| Applications
|
| Application | The sensitivity of Single Molecule Counting (SMC™) technology gives the Total GLP-1 Immunoassay Kit unparalleled performance in the quantification of total GLP-1 in human EDTA plasma. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
|
| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
|
| Physicochemical Information
|
| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96 well assay |
