millipore,
密理博,03-0006-03,SMC Insulin (Mouse, Plate-based) Immunoassay KitIMMUNOASSAY KIT
重要规格表
| Analytes Available | Species Reactivity | Key Applications |
| Insulin
| M
| Single Molecule Counting (SMC™)
|
| Description
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| Catalogue Number | 03-0006-03 |
| Trade Name |
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| Description | SMC™ Insulin (Mouse, Plate-based) Immunoassay Kit IMMUNOASSAY KIT |
| Overview | The SMC® Mouse Plate-based Insulin Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Insulin is an important metabolic hormone produced by islet cells within the pancreas whose primary function is to regulate blood sugar levels in the body. When blood glucose level is high (ie. after food ingestion), insulin increases glucose uptake and forces liver cells to convert glucose to glycogen for storage. Insulin is also known to promote glycolysis, fatty acid synthesis and amino acid uptake. Patients who do not produce sufficient insulin (Type 1) or become resistant to insulin (Type 2) develop diabetes mellitus and suffer from hyperglycemia, which can lead to complications such as blindness, cardiovascular disease, and kidney damage. Many insulin analogs, which mimic either normal or rapid-acting (ie.lispro) insulin functions have been developed as treatment for diabetes. |
| Product Information
|
| Components |
- Mouse Insulin Assay Plate
- Mouse Insulin Detection Reagent
- Mouse Insulin Standard (frozen, shipped in separate box)
- Mouse Insulin Standard Diluent
- 10X Wash Buffer
- Elution Buffer (insulin free)
- SMC™ Insulin Mouse Immunoassay Kit Instructions
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| Precision, % |
- Low-end CV% Range: 3 - 16%
- Low-end CV% Average: 13%
|
| Quality Level | MQ300
|
| Applications
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| Application | The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Mouse Plate-based Insulin Immunoassay Kit unparalleled performance in the quantification of insulin in mouse heparin plasma. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Biological Information
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| Species Reactivity |
|
| Analytes Available |
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| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
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| Physicochemical Information
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| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Packaging Information
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| Material Size | 96 well assay |