重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|---|---|---|
| ARG1, GOT1, GSTα, SDH, 5'-NT/CD73 | R | Mplex | Luminex xMAP |
| Description | |
|---|---|
| Catalogue Number | RLI1MAG-92K |
| Trade Name |
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| Description | MILLIPLEX MAP Rat Liver Injury Panel - Toxicity Multiplex Assay |
| Overview | The MILLIPLEX® MAP Rat Liver Injury Magnetic Bead Panel (RLI1MAG-92K) contains all the components necessary to simultaneous quantify the following 5 analytes in serum and plasma samples: • Liver-Type Arginase 1 (ARG1)* • Aspartate transaminase 1 (GOT1) • α-glutathione S-transferase (GSTα)* • Sorbitol Dehydrogenase (SDH)* • 5'-Nucleotidase/CD73 (5’-NT) *ARG1, GSTα, and SDH are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. EMD millipore provides a valuable research assay to investigate multiple biomarkers of liver injury in rat serum and plasma samples using the Luminex xMAP® instrument platform. This kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum or plasma samples in duplicate. |
| Background Information | Drug toxicity is the leading cause of acute liver failure in the United States. Patients with liver damage generally display elevated amounts of specific liver proteins in serum; these proteins can serve as biomarkers of drug-related liver toxicity. Monitoring these biomarkers can greatly help clinicians avoid drug-induced liver failure. Performing laboratory tests to characterize the side effects of potential therapeutics is an essential part of drug development. The rat is a leading animal model for these pre-clinical toxicity studies. The search for sensitive, organ-specific toxicity biomarkers is complemented by the development of novel assays to measure these critical analytes. Liver-Type Arginase 1 (ARG1), α-glutathione S-transferase (GSTα), and Sorbitol Dehydrogenase (SDH) are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. Aspartate transaminase 1 (GOT1) and 5'-Nucleotidase / CD73 (5’-NT) are traditional biomarkers recognized by both the Food and Drug Administration (FDA) and its European counterpart, the European Medicines Agency (EMA). |
| Product Information | |
|---|---|
| Components |
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
|
| Panel Type | MAGNETIC Toxicity |
| Quality Level | MQ200 |
| Applications | |
|---|---|
| Application | The analytes available for this multiplex kit are: Liver-type arginase 1 (ARG1), aspartate transaminase 1 (GOT1), α-glutathianone S-transferase (GSTα), sorbitol dehydrogenase (SDH), and 5'-Nucleotidase (5'-NT/CD73). |
| Key Applications |
|
| Application Notes | • This is an overnight assay. • This assay requires 25 µL of 1:25 diluted serum or plasma sample per well in kit Assay Buffer (please dilute per protocol instructions). |
| Biological Information | |
|---|---|
| Specificity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Species Reactivity |
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| Analytes Available |
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| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Accuracy |
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| Physicochemical Information | |
|---|---|
| Sensitivity |
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| Linearity of Dilution | Contact technical support for this information. |
| Standard Curve Range |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information | |
|---|---|
| Material Size | 96-well plate |
