millipore,
密理博,PCYTMG-40K-PX23,MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| G-CSF, GM-CSF, IFN-γ, IL-1ra, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12/23 (p40), IL-13, IL-15, IL-17, IL-18, MCP-1, MIP-1α, MIP-1β, sCD40L, TGF-α, TNF-α, VEGF
| NhP
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | PCYTMG-40K-PX23 |
| Trade Name |
|
| Description | MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology Multiplex Assay |
| Overview | To identify specific cytokines involved in any inflammatory or immune response, it may be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing
Therefore, the MILLIPLEX MAP Non-Human Primate Cytokine / Chemokine Panel enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improve productivity. |
| Background Information | The immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Premixed |
| Precision, % |
- Inter-assay: 8 - 15
- Intra-assay: 2 - 5
|
| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200
|
| Applications
|
| Application | Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology in non-human primate serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | • This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• This assay requires 25 µl of neat plasma or serum, or 25 μL cell culture supernatant per well. |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
- G-CSF
- GM-CSF
- IFN-γ
- IL-1ra
- IL-1β
- IL-2
- IL-4
- IL-5
- IL-6
- IL-8
- IL-10
- IL-12/23 (p40)
- IL-13
- IL-15
- IL-17
- IL-18
- MCP-1
- MIP-1α
- MIP-1β
- sCD40L
- TGF-α
- TNF-α
- VEGF
|
| Assay Panel | Premixed beads for your convenience. |
| Cross Reactivity | The antibody pairs in the panel are specific only to the desired analyte and exhibit no or negligible cross-reactivity with other analytes in the panel |
| Accuracy |
|
| Physicochemical Information
|
| Sensitivity |
- Refer to kit protocol for sensitivities for individual cytokines/chemokines
|
| Linearity of Dilution | 1:6 110.3% 1:24 126.2% 1:12 121.6% |
| Standard Curve Range |
- IL-4: 4.9 - 20,000 pg/mL
- IL-10, IL-18: 12.2 - 50,000 pg/mL
- All other analytes: 2.4 - 10,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
