| Description
|
| Overview | Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for bovine, goat, human, and rat IgG and for mouse IgG1. |
| Catalogue Number | IP04 |
| Brand Family |
Calbiochem®
|
| Product Information
|
| Form | Liquid slurry |
| Formulation | In PBS. |
| Preservative | ≤0.1% sodium azide |
| Applications
|
| Key Applications |
Immunoprecipitation
|
| Application Notes | Immunoprecipitation (see comments) |
| Application Comments | Agarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody. |
| Storage and Shipping Information
|
| Ship Code |
Blue Ice Only
|
| Toxicity |
Standard Handling
|
| Storage |
+2°C to +8°C
|
| Do not freeze |
Yes
|
