millipore,
密理博,ES009-500ML,p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble)
重要规格表
| Description
|
| Catalogue Number | ES009-500ML |
| Brand Family |
Chemicon®
|
| Trade Name |
|
| Description | p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble) |
| Product Information
|
| Presentation | p-NPP, a single component substrate in proprietary stabilizer. |
| Color Code | Yellow |
| Applications
|
| Application | 500 ml p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble) for ELISA. |
| Key Applications |
|
| Application Notes | p-Nitrophenyl phosphate (p-NPP) is the customary product utilized for the assay of alkaline phosphatase. It is usually supplied in tablet or powder form and is dissolved in an appropriate buffer immediately prior to assay. Liquid p-NPP concentrates are also available, but must also be diluted in buffer. CHEMICON, Inc. now provides p-NPP stabilized in diethanolamine (DEA) buffer and available for instant use. The DEA buffer contains Mg++ and Zn++ ions providing optimum activation of alkaline phosphatase labeled probes.
This is an excellent substrate for phosphatase based ELISA assays.
It produces a soluble end product which is yellow in color and reads at 405 nm to 410 nm. Both kinetic and end point determinations can be performed. |
| Physicochemical Information
|
| Quantitation Wavelength | 405 nm |
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Maintain at 2-8°C for up to one year. |
| Packaging Information
|
| Material Size | 500 mL |
