millipore,
密理博,539180,ProteoExtract Protein Precipitation Kit
| Description
|
| Overview | A convenient and highly reproducible kit for the precipitation and clean-up of proteins. Yield of precipitated proteins is higher than that of traditional methods with improved solubility of the precipitated protein. Contains sufficient components to precipitate 200 samples of ≤200 µl. Requires 150 ml of ethanol (not provided). Kit delivers protein solutions of very low conductivity. |
| Catalogue Number | 539180 |
| Brand Family |
Calbiochem®
|
| Application Data | 
The ProteoExtract® Protein Precipitation Kit yields protein samples with significantly lower conductivity then other precipitation methods.
Proteins at a concentration of 1 mg/ml were precipitated from 1 M sodium chloride solutions according to the Detailed Protocol. The same experiment was performed using standard protocols as TCA/DOC or acetone precipitation. Protein pellets were solubilized in IEF buffer for conductivity measurement. |
| Materials Required but Not Delivered | • Ethanol
• Microcentrifuge
• Micropipettes and tips, 10 µl, 200 µl and 1 ml size |
| Product Information
|
| Form | 200 Precipitations |
| Kit contains | Precipitant 1, Precipitant 2, Precipitant 3, Precipitant 4, Wash Solution, and a user protocol. |
| Product Usage Statements
|
| Intended use | The ProteoExtract® Protein Precipitation Kit is designed for the concentration and clean-up of proteins from aqueous samples. |
| Storage and Shipping Information
|
| Ship Code |
Ambient Temperature Only
|
| Toxicity |
Multiple Toxicity Values, refer to MSDS
|
| Hazardous Materials Attention: |
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges.
|
| Storage |
+15°C to +30°C
|
| Storage Conditions | Upon arrival, all components of the kit can be stored at room temperature (20°C). For ease of use, the prepared Precipitation Agent and the Wash Solution can be stored at -20°C (see Reagent Preparation). |
| Do not freeze |
Ok to freeze
|
| Supplemental Information
|
| Kit contains | Precipitant 1, Precipitant 2, Precipitant 3, Precipitant 4, Wash Solution, and a user protocol. |
