millipore,
密理博,48-630MAG,MILLIPLEXMAP NF-κB Signaling Magnetic Bead Kit 6-plex Kit - Cell Signaling Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| c-Myc, FADD (Ser194), IκBα (Ser32), IKKα/β (Ser177/Ser181), NFκB (Ser536), TNFR1
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | 48-630MAG |
| Trade Name |
|
| Description | MILLIPLEXMAP NF-κB Signaling Magnetic Bead Kit 6-plex Kit - Cell Signaling Multiplex Assay |
| Overview | NF-κB (nuclear factor kappa B) is a transcription factor that plays a regulatory role in several physiological and pathological processes. The NF-κB pathway can be activated by numerous stimuli, including inflammatory cytokines, virus, bacteria, UV radiation, genotoxic stress, DNA damage, oxidative stress, etc. The IkBα/NF-κB complex is tightly regulated by both phosphorylation and ubiquitination. Phosphorylation and ubiquitination of IκBα leads to its degradation and subsequent release of phosphorylated NF-κB to the nucleus. Greater than 100 target genes, including cytokines, cyclins, p21, Bcl-2, and c-Myc are known to be transcribed by NF-kB. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Premixed |
| Panel Type | Cell Signaling |
| Quality Level | MQ200
|
| Applications
|
| Application | Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting. |
| Key Applications |
|
| Application Notes | o This is an overnight (4°C) incubation assay.
o This kit must be run using the provided Assay Buffer.
o This assay requires 25 μL diluted filtered cell lysate per well.
o The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL). |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
- c-Myc
- FADD (Ser194)
- IκBα (Ser32)
- IKKα/β (Ser177/Ser181)
- NFκB (Ser536)
- TNFR1
|
| Assay Panel | Premixed beads for your convenience. |
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96-well plate |
