millipore,
密理博,48-618MAG,MILLIPLEX MAP Total/Phospho Akt - Cell Signaling Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| Akt (Total), Akt (Ser473)
| H, M, R
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | 48-618MAG |
| Trade Name |
|
| Description | MILLIPLEX MAP Total/Phospho Akt - Cell Signaling Multiplex Assay |
| Overview | The MILLIPLEX® MAP Total/Phospho Akt (48-618MAG) and Total/Phospho ERK (Catalog # 48-619MAG) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Premixed |
| Panel Type | Cell Signaling |
| Quality Level | MQ200
|
| Applications
|
| Application | Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples. |
| Key Applications |
|
| Application Notes | • An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL) |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96-well plate |
