millipore,
密理博,47-229,MILLIPLEX MAP HeLa Cell Lysate: Lambda Phosphatase - Lysates/Reagents for Multiplex Assays
重要规格表
| Key Applications |
|---|
| Mplex, WB
|
| Description
|
| Catalogue Number | 47-229 |
| Trade Name |
|
| Description | MILLIPLEX MAP HeLa Cell Lysate: Lambda Phosphatase - Lysates/Reagents for Multiplex Assays |
| Overview | One vial containing 200 μg of total proteins lyophilized from 100 μL of cell lysate made from HeLa cells that were lysed in MILLIPLEX MAP Lysis Buffer with protease inhibitors and treated with lambda phosphatase (1,000U/mg total protein for 30 min. at 37֯°C). |
| Product Information
|
| Detection method | Luminex xMAP Fluorescent |
| Quality Level | MQ200
|
| Applications
|
| Application | This MILLIPLEX MAP HeLa Cell Lysate: Lambda Phosphatase, is a control lysate used for multiplex Cell Signaling bead-based kits. |
| Key Applications |
- Multiplexing
- Western Blotting
|
| Application Notes | HeLa Cell Lysate: Lambda Phosphatase is recommended for use in Western blotting (starting at 20 – 40 μg/well), immunoprecipitation (starting at 40 – 100 μg per sample), and Luminex® bead assays (starting at 2 - 20 μg/well) |
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Store at 2-8°C. |
| Packaging Information
|
| Material Size | 100 µL |
