millipore,密理博,46-675MAG,MILLIPLEX MAP Total Akt/PKB MAPmate Assay - Cell Signaling Single Plex or Multiplex Assay
| Description | |
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| Catalogue Number | 46-675MAG |
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| Description | MILLIPLEX MAP Total Akt/PKB MAPmate Assay - Cell Signaling Single Plex or Multiplex Assay |
| Materials Required but Not Delivered | • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602) |
| Product Information | |
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| Detection method | Luminex xMAP |
| Configuration | Single Plex |
| Panel Type | Cell Signaling |
| Quality Level | MQ200 |
| Applications | |
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| Application | MAPmate single plexes allow you to design your own cell signaling panel. The analyte available for this kit is Akt (Total). |
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| Application Notes | • An overnight (4°C) incubation is recommended for best results • This assay requires 25 µL diluted cell lysate per well • Please consult the buffer compatibility chart or protocol for appropriate buffer choice • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg) |
| Biological Information | |
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| Species Reactivity |
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| Entrez Gene Number |
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| Entrez Gene Summary | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. |
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| UniProt Summary | FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. SIZE: 480 amino acids; 55686 Da SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). TISSUE SPECIFICITY: In all human cell types so far analyzed. DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane. PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A). SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
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| Storage and Shipping Information | |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information | |
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| Material Size | 96-well plate |
| Material Package | 96-well Plate |
