millipore,密理博,400050,Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem
重要规格表
| Empirical Formula | CAS # |
|---|---|
| C₂₀H₃₇N₃O₁₃ | 31282-04-9 |
| Description | |
|---|---|
| Overview | Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection. |
| Potency: ≥1000 µ/mg solid. | |
| Catalogue Number | 400050 |
| Brand Family | Calbiochem® |
| References | |
|---|---|
| References | Dean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287. Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717. Rikkerink, E. H., et al. 1994. Current Genetics 25, 202. Sugimoto, K., et al. 1994. Plant J. 5, 863. MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67. Bilang, R., et al. 1991. Gene 100, 247. Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255. Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423. MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125. Zhou, J., et al. 1991. Gene 107, 307. Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155. Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285. Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915. Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311. Crespi, C.L., et al. 1989. Carcinogenesis 10, 295. Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6. Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965. Kronstad, J.W., et al. 1989. Gene 79, 97. Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118. Cullen, D., et al. 1987. Gene 57, 21. Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459. Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6. Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689. |
| Product Information | |
|---|---|
| CAS number | 31282-04-9 |
| Form | Light brown lyophilized solid |
| Hill Formula | C₂₀H₃₇N₃O₁₃ |
| Chemical formula | C₂₀H₃₇N₃O₁₃ |
| Structure formula Image |  |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application Comments | Provided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use. |
| Biological Information | |
|---|---|
| Biological activity | The activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard. |
| Purity | ≥90% by HPLC and TLC |
| Safety Information according to GHS | |
|---|---|
| RTECS | WK2130000 |
| Safety Information | |
|---|---|
| R Phrase | R: 26/27/28-37/38-41 Very toxic by inhalation, in contact with skin and if swallowed. Irritating to respiratory system and skin. Risk of serious damage to eyes. |
| S Phrase | S: 22-26-36/37/39-45 Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
| Storage and Shipping Information | |
|---|---|
| Ship Code | Ambient Temperature Only |
| Toxicity | Highly Toxic & Carcinogenic / Teratogenic |
| Hazardous Materials Attention: | Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. |
| Storage | +2°C to +8°C |
| Do not freeze | Ok to freeze |
| Special Instructions | Aqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C. Note: This product is highly toxic. Read enclosed MSDS before handling. |
