| Appearance (clearness) | clear |
| Appearance (color) | yellowish to yellowish-braun |
| pH-value (25 °C) | 6.7 - 7.1 |
| Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)) | ≤ 100 |
| Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090)) | ≤ 100 |
| Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | ≥ 10000 |
| Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | ≥ 10000 |
| Inoculum on reference medium (Proteus hauseri ATCC 13315) | ≥ 10000 |
| Growth (Escherichia coli ATCC 35150 (O157)) | > 10 transparent colonies with a pale yellowish-brown appearance on SMAC agar
|
| Growth (Escherichia coli ATCC 11775 (WDCM 00090)) | > 10 pink colonies on SMAC Agar |
| Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | total inhibition on TSA |
| Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | total inhibition on TSA |
Mixed inoculum of E.coli, P.hauseri and E.faecalis
Additionaly monoculture of E.faecalis
Confirmation on SMAC agar resp. Tryptic soy agar
Incubation:
mEC broth: 18 - 24 hours at 41,5 ± 1 °C aerobic
Confirmation on SMAC agar resp. Tryptic Soy Agar: 18 - 24 hours at 35 ± 1 °C aerobic
|
