millipore,
密理博,03-0132-00,SMC Mouse IL-5 Discovery Immunoassay Kit
重要规格表
| Analytes Available | Species Reactivity | Key Applications |
|---|
| IL-5
| M
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0132-00 |
| Trade Name |
|
| Description | SMC™ Mouse IL-5 Discovery Immunoassay Kit |
| Overview | The SMC™ Mouse IL-5 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Interleukin-5 (IL-5) is a cytokine secreted mainly by T-cells and mast cells upon stimulation with antigens and allergens. It has pleiotropic effects toward B-cells and is involved in the production of immunoglobulins. More importantly, IL-5 acts in synergy with GM-CSF to govern the proliferation and maturation of eosinophils. A pronounced level of eosinophils is commonly associated with the development of allergic conditions such as asthma, eosinophilic esophagitis and hypereosinophilic syndrome. As such, IL-5 is highly regarded as a crucial mediator of the inflammatory pathway concerning allergic reactions. Current studies suggest that IL-5 blockade is a promising intervention for the treatment of allergic diseases. |
| Product Information
|
| Components |
- Assay Buffer
- Mouse IL-5 Coating Antibody
- Assay Plate
- 10X PBS
- Blocking Buffer
- Standard Diluent
- Mouse IL-5 Detection Antibody
- Mouse IL-5 Standard
- SMC™ Mouse IL-5 Immunoassay Kit Instructions
- 10X Wash Buffer
- Buffer C
- Elution Buffer B
|
| Precision, % |
- Low-end CV% Range: 0-19%
- Low-end CV% Average: 6.5%
|
| Quality Level | MQ200
|
| Applications
|
| Application | The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Mouse IL-5 Discovery Immunoassay unparalleled performance in the quantification of IL-5 in mouse serum or EDTA plasma. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
|
| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
|
| Physicochemical Information
|
| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96 well assay |
